WORKLIST ENTRIES (1):

MAPKPHPHTASE View alignment View Structure    MAP kinase phosphatase signature
 Type of fingerprint: COMPOUND with 5  elements
Links:
   PRINTS; PR00700 PRTYPHPHTASE
   PDB; 1HZM 3Dinfo; 1VHR 3Dinfo
   SCOP; 1HZM; 1VHR
   CATH; 1HZM; 1VHR

 Creation date 18-JUL-2002

   1. SUN, H., CHARLES, C.H., LAU, L.F. AND TONKS, N.K. 
   MKP-1 (3CH134), an immediate-early gene-product is a dual specificity
   phosphatase that dephosphorylates MAP kinase in-vivo. 
   CELL 75(3) 487-493 (1993).

   2. MUDA, M., THEODOSIOU, A., RODRIGUES, N., BOSCHERT, U., CAMPS, M., 
   GILLIERON, C., DAVIES, K., ASHWORTH, A. AND ARKINSTALL, S.
   The dual specificity phosphatases M3/6 and MKP-3 are highly selective for 
   inactivation of distinct mitogen activated protein kinases. 
   J.BIOL.CHEM. 271(44) 27205-27208 (1996).

   3. DIXON, J.E. AND SAPER, M.A.
   Crystal structure of the dual specificity protein phosphatase VHR.
   SCIENCE 272 1328-1331 (1996).

   The dual specificity phosphatases, also known as MAP Kinase Phosphatases 
   (MKP), constitute a class of phosphatases that reverse the activation of MAP
   (mitogen activated protein) kinases by dephosphorylating critical tyrosine 
   and threonine residues [1]. This regulation is mediated via interaction of a 
   Kinase Interaction Motif (KIM) with the common docking domain of the kinase 
   - this motif is shared with a number of other protein families that interact 
   with MAP kinases: these include kinases (MEKs), phosphatases (PTP-SL) and 
   transcription factors (Elk1). MKPs also share an active site motif with the
   protein tyrosine phosphatases (PTPs). Different MKPs exhibit specificity 
   towards different members of the MAP kinase family [2].
  
   The structure of the MPK catalytic domain is similar to that of the PTPs 
   [3] - the fold exhibits a complex mixed alpha-/beta-architecture. The 
   catalytic mechanism involves a general acid (Asp92 in 1VHR) and a catalytic 
   cysteine (130 in 1VHR), which acts as a nucleophile.
  
   MAPKPHPHTASE is a 5-element fingerprint that provides a signature for the 
   MAP kinase phosphatases. The fingerprint was derived from an initial 
   alignment of 12 sequences: the motifs were drawn from conserved regions 
   spanning the full alignment length. Two iterations on SPTR40_20f were
   required to reach convergence, at which point a true set comprising 30 
   sequences was identified. Several partial matches were also found, most of
   which appear to be N- or C-terminal fragments.

  SUMMARY INFORMATION
     30 codes involving  5 elements
      0 codes involving  4 elements
      5 codes involving  3 elements
      2 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    5|  30   30   30   30   30  
    4|   0    0    0    0    0  
    3|   0    0    5    5    5  
    2|   1    1    1    0    1  
   --+--------------------------
     |   1    2    3    4    5  

True positives..
 Q64193         Q63683         DUS1_RAT       DUS1_MOUSE     
 DUS1_HUMAN     Q90W58         Q91790         DUS4_CHICK     
 DUS4_HUMAN     DUS4_RAT       DUS2_MOUSE     DUS2_HUMAN     
 Q96N49         Q920R2         Q9AG16         Q9AG15         
 Q99MG6         Q99MG5         DUS9_HUMAN     DUS6_HUMAN     
 DUS6_RAT       Q99KC2         DUS5_RAT       DUS5_HUMAN     
 DUS8_MOUSE     Q91663         DUS8_HUMAN     Q8UW48         
 Q9ESS0         Q9CZY9         
Subfamily:  Codes involving 3 elements
 Subfamily True positives..
 Q9NSW1         Q8WXV0         Q9H9Z5         Q95SV1         
 Q9VVW4         
Subfamily:  Codes involving 2 elements
 Subfamily True positives..
 Q96QS2         O44128         


  PROTEIN TITLES
   Q64193           3CH134/CL100 PTPASE - Rattus sp.
   Q63683           PROTEIN TYROSINE PHOSPHATASE - Rattus norvegicus (Rat).
   DUS1_RAT         Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1
   DUS1_MOUSE       Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1
   DUS1_HUMAN       Dual specificity protein phosphatase 1 (EC 3.1.3.48) (EC 3.1
   Q90W58           MAP KINASE PHOSPHATASE XCL100(BETA) PROTEIN - Xenopus laevis
   Q91790           DUAL SPECIFICITY PROTEIN PHOSPHATASE XCL100 (EC 3.1.3.48) (E
   DUS4_CHICK       Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1
   DUS4_HUMAN       Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1
   DUS4_RAT         Dual specificity protein phosphatase 4 (EC 3.1.3.48) (EC 3.1
   DUS2_MOUSE       Dual specificity protein phosphatase 2 (EC 3.1.3.48) (EC 3.1
   DUS2_HUMAN       Dual specificity protein phosphatase 2 (EC 3.1.3.48) (EC 3.1
   Q96N49           CDNA FLJ31411 FIS, CLONE NT2NE2000214, MODERATELY SIMILAR TO
   Q920R2           MAP KINASE PHOSPHATASE-7 - Mus musculus (Mouse).
   Q9AG16           MAP KINASE PHOSPHATASE-M B1 ISOFORM - Mus musculus (Mouse).
   Q9AG15           MAP KINASE PHOSPHATASE-M B2 ISOFORM - Mus musculus (Mouse).
   Q99MG6           MAP KINASE PHOSPHATASE-M A1 ISOFORM - Mus musculus (Mouse).
   Q99MG5           MAP KINASE PHOSPHATASE-M A2 ISOFORM - Mus musculus (Mouse).
   DUS9_HUMAN       Dual specificity protein phosphatase 9 (EC 3.1.3.48) (EC 3.1
   DUS6_HUMAN       Dual specificity protein phosphatase 6 (EC 3.1.3.48) (EC 3.1
   DUS6_RAT         Dual specificity protein phosphatase 6 (EC 3.1.3.48) (EC 3.1
   Q99KC2           SIMILAR TO DUAL SPECIFICITY PHOSPHATASE 9 - Mus musculus (Mo
   DUS5_RAT         Dual specificity protein phosphatase 5 (EC 3.1.3.48) (EC 3.1
   DUS5_HUMAN       Dual specificity protein phosphatase 5 (EC 3.1.3.48) (EC 3.1
   DUS8_MOUSE       Dual specificity protein phosphatase 8 (EC 3.1.3.48) (EC 3.1
   Q91663           DUAL SPECIFICITY PROTEIN PHOSPHATASE ? (EC 3.1.3.48) (EC 3.1
   DUS8_HUMAN       Dual specificity protein phosphatase 8 (EC 3.1.3.48) (EC 3.1
   Q8UW48           DUAL SPECIFICITY PHOSPHATASE 6, ISOFORM A - Fugu rubripes (J
   Q9ESS0           MAP KINASE PHOSPHATASE-5 - Mus musculus (Mouse).
   Q9CZY9           2610306G15RIK PROTEIN - Mus musculus (Mouse).
 
   Q9NSW1           HYPOTHETICAL 33.0 KDA PROTEIN - Homo sapiens (Human).
   Q8WXV0           PHOSPHATASE - Homo sapiens (Human).
   Q9H9Z5           CDNA FLJ12451 FIS, CLONE NT2RM1000377, HIGHLY SIMILAR TO HOM
   Q95SV1           SD06439P - Drosophila melanogaster (Fruit fly).
   Q9VVW4           CG14080 PROTEIN - Drosophila melanogaster (Fruit fly).
 
   Q96QS2           TRUNCATED MAPK PHOSPHATASE 7 - Homo sapiens (Human).
   O44128           C05B10.1 PROTEIN - Caenorhabditis elegans.

SCAN HISTORY SPTR40_20f 2 75 NSINGLE INITIAL MOTIF SETS MAPKPHPHTASE1 Length of motif = 11 Motif number = 1 MAP kinase phosphatase motif I - 1 PCODE ST INT LLLDCRPFLAF DUS2_HUMAN 28 28 VVLDCRPYLAF DUS5_RAT 24 24 LLLDCRPFLAH DUS4_HUMAN 46 46 LLLDCRPFLAH DUS4_RAT 47 47 LLLDCRSFFAF DUS1_HUMAN 25 25 LLLDCRSFFAF DUS1_MOUSE 25 25 LLLDCRSFFAF DUS1_RAT 25 25 LLLDCRSRELY DUS9_HUMAN 23 23 LILDCRSFFAF Q90W58 26 26 LLLDCRSFFAF Q64193 25 25 LLMDCRPQELY DUS6_HUMAN 35 35 LLMDCRPQELY DUS6_RAT 35 35 MAPKPHPHTASE2 Length of motif = 13 Motif number = 2 MAP kinase phosphatase motif II - 1 PCODE ST INT ALLRRRARGPPAA DUS2_HUMAN 53 14 VVLRRARGGAVSA DUS5_RAT 50 15 TIVRRRAKGSVSL DUS4_HUMAN 71 14 TIVRRRAKGSVSL DUS4_RAT 72 14 TIVRRRAKGAMGL DUS1_HUMAN 50 14 TIVRRRAKGAMGL DUS1_MOUSE 50 14 TIVRRRAKGAMGL DUS1_RAT 50 14 LLLRRLRRGSLSV DUS9_HUMAN 49 15 TIVKRRAKGSMGL Q90W58 51 14 TIVRRRAKGAMGL Q64193 50 14 IMLRRLQKGNLPV DUS6_HUMAN 61 15 IMLRRLQKGNLPV DUS6_RAT 61 15 MAPKPHPHTASE3 Length of motif = 11 Motif number = 3 MAP kinase phosphatase motif III - 1 PCODE ST INT ITAVLNVSASC DUS2_HUMAN 199 133 ITALLNVSRRT DUS5_RAT 205 142 ITALLNVSSDC DUS4_HUMAN 222 138 ITALLNVSSDC DUS4_RAT 223 138 ITALINVSANC DUS1_HUMAN 200 137 ITALINVSANC DUS1_MOUSE 200 137 ITALINVSANC DUS1_RAT 200 137 IRYILNVTPNL DUS9_HUMAN 230 168 ITALINVSANC Q90W58 202 138 ITALINVSANC Q64193 200 137 IKYILNVTPNL DUS6_HUMAN 233 159 IKYILNVTPNL DUS6_RAT 233 159 MAPKPHPHTASE4 Length of motif = 10 Motif number = 4 MAP kinase phosphatase motif IV - 1 PCODE ST INT YKSIPVEDNQ DUS2_HUMAN 219 9 YKWIPVEDSH DUS5_RAT 225 9 YKCIPVEDNH DUS4_HUMAN 242 9 YKCIPVEDNH DUS4_RAT 243 9 YKSIPVEDNH DUS1_HUMAN 220 9 YKSIPVEDNH DUS1_MOUSE 220 9 YKSIPVEDNH DUS1_RAT 220 9 YKQIPISDHW DUS9_HUMAN 252 11 YKSIPVEDSH Q90W58 222 9 YKSIPVEDNH Q64193 220 9 YKQIPISDHW DUS6_HUMAN 255 11 YKQIPISDHW DUS6_RAT 255 11 MAPKPHPHTASE5 Length of motif = 13 Motif number = 5 MAP kinase phosphatase motif V - 1 PCODE ST INT WFQEAIGFIDWVK DUS2_HUMAN 235 6 HFQEAIDFIDCVR DUS5_RAT 241 6 WFMEAIEYIDAVK DUS4_HUMAN 258 6 WFMEAIEYIDAVK DUS4_RAT 259 6 WFNEAIDFIDSIK DUS1_HUMAN 236 6 WFNEAIDFIDSIK DUS1_MOUSE 236 6 WFNEAIDFIDSIK DUS1_RAT 236 6 FFPEAIEFIDEAL DUS9_HUMAN 268 6 WFNEAIDFIDSIK Q90W58 238 6 WFNEAIDFIDSIK Q64193 236 6 FFPEAISFIDEAR DUS6_HUMAN 271 6 FFPEAISFIDEAR DUS6_RAT 271 6 FINAL MOTIF SETS MAPKPHPHTASE1 Length of motif = 11 Motif number = 1 MAP kinase phosphatase motif I - 2 PCODE ST INT LLLDCRSFFAF DUS1_HUMAN 25 25 LLLDCRSFFAF DUS1_MOUSE 25 25 LLLDCRSFFAF DUS1_RAT 25 25 LLLDCRSFFAF Q63683 25 25 LLLDCRSFFAF Q64193 25 25 LILDCRSFFAF Q90W58 26 26 LILDCRSFFSF Q91790 26 26 LLLDCRPFLAH DUS4_CHICK 30 30 LLLDCRPFLAH DUS4_HUMAN 46 46 LLLDCRPFLAH DUS4_RAT 47 47 LLLDCRPFLAF DUS2_MOUSE 32 32 LLLDCRPFLAF DUS2_HUMAN 28 28 LLIDSRPFVEY Q920R2 27 27 LLIDSRPFVEY Q96N49 27 27 LLIDSRPFVEY Q99MG5 27 27 LLIDSRPFVEY Q99MG6 27 27 LLIDSRPFVEY Q9AG15 27 27 LLIDSRPFVEY Q9AG16 27 27 LLLDCRSRELY DUS9_HUMAN 23 23 LLMDCRPQELY DUS6_HUMAN 35 35 LLMDCRPQELY DUS6_RAT 35 35 LLLDCRSRELY Q99KC2 23 23 VVLDCRPYLAF DUS5_HUMAN 24 24 VVLDCRPYLAF DUS5_RAT 24 24 LVIDSRSFVEY DUS8_MOUSE 28 28 LLMDCRAHELY Q91663 34 34 LVIDSRSFVEY DUS8_HUMAN 28 28 LVMDCRAQELY Q8UW48 36 36 VIIDCRPFMEY Q9CZY9 174 174 VIIDCRPFMEY Q9ESS0 174 174 MAPKPHPHTASE2 Length of motif = 13 Motif number = 2 MAP kinase phosphatase motif II - 2 PCODE ST INT TIVRRRAKGAMGL DUS1_HUMAN 50 14 TIVRRRAKGAMGL DUS1_MOUSE 50 14 TIVRRRAKGAMGL DUS1_RAT 50 14 TIVRRRAKGAMGL Q63683 50 14 TIVRRRAKGAMGL Q64193 50 14 TIVKRRAKGSMGL Q90W58 51 14 TIVKRRAKGSMGL Q91790 51 14 TIVRRRAKGAVSL DUS4_CHICK 55 14 TIVRRRAKGSVSL DUS4_HUMAN 71 14 TIVRRRAKGSVSL DUS4_RAT 72 14 ALLRRRAPGTPAA DUS2_MOUSE 57 14 ALLRRRARGPPAA DUS2_HUMAN 53 14 KLMKRRLQQDKVL Q920R2 52 14 KLMKRRLQQDKVL Q96N49 52 14 KLMKRRLQQDRVL Q99MG5 52 14 KLMKRRLQQDRVL Q99MG6 52 14 KLMKRRLQQDRVL Q9AG15 52 14 KLMKRRLQQDRVL Q9AG16 52 14 LLLRRLRRGSLSV DUS9_HUMAN 49 15 IMLRRLQKGNLPV DUS6_HUMAN 61 15 IMLRRLQKGNLPV DUS6_RAT 61 15 LMLRRLRRGSMSV Q99KC2 49 15 VVLRRARGGAVSA DUS5_HUMAN 50 15 VVLRRARGGAVSA DUS5_RAT 50 15 KLVKRRLQQGKVT DUS8_MOUSE 53 14 IMLRRLKKGNLPI Q91663 60 15 KLVKRRLQQGKVT DUS8_HUMAN 53 14 LMLRRLKKGNLPV Q8UW48 62 15 KISRRRLQQGKIT Q9CZY9 200 15 KISRRRLQQGKIT Q9ESS0 200 15 MAPKPHPHTASE3 Length of motif = 11 Motif number = 3 MAP kinase phosphatase motif III - 2 PCODE ST INT ITALINVSANC DUS1_HUMAN 200 137 ITALINVSANC DUS1_MOUSE 200 137 ITALINVSANC DUS1_RAT 200 137 ITALINVSANC Q63683 200 137 ITALINVSANC Q64193 200 137 ITALINVSANC Q90W58 202 138 ITALINVSANC Q91790 202 138 ITALLNVSSDC DUS4_CHICK 203 135 ITALLNVSSDC DUS4_HUMAN 222 138 ITALLNVSSDC DUS4_RAT 223 138 ITAVLNVSASC DUS2_MOUSE 203 133 ITAVLNVSASC DUS2_HUMAN 199 133 IGYVLNASNTC Q920R2 185 120 IGYVLNASNTC Q96N49 185 120 IGYVLNASNTC Q99MG5 130 65 IGYVLNASNTC Q99MG6 185 120 IGYVLNASNTC Q9AG15 130 65 IGYVLNASNTC Q9AG16 185 120 IRYILNVTPNL DUS9_HUMAN 230 168 IKYILNVTPNL DUS6_HUMAN 233 159 IKYILNVTPNL DUS6_RAT 233 159 IRYILNVTPNL Q99KC2 282 220 ITALLNVSRRT DUS5_HUMAN 205 142 ITALLNVSRRT DUS5_RAT 205 142 ISYVLNASNSC DUS8_MOUSE 187 121 IKYILNVTPNL Q91663 230 157 ISYVLNASNSC DUS8_HUMAN 187 121 IKYILNVTPNL Q8UW48 233 158 IGYVINVTTHL Q9CZY9 349 136 IGYVINVTTHL Q9ESS0 349 136 MAPKPHPHTASE4 Length of motif = 10 Motif number = 4 MAP kinase phosphatase motif IV - 2 PCODE ST INT YKSIPVEDNH DUS1_HUMAN 220 9 YKSIPVEDNH DUS1_MOUSE 220 9 YKSIPVEDNH DUS1_RAT 220 9 YKSIPVEDNH Q63683 220 9 YKSIPVEDNH Q64193 220 9 YKSIPVEDSH Q90W58 222 9 YKSIPVEDSH Q91790 222 9 YKCIPVEDNH DUS4_CHICK 223 9 YKCIPVEDNH DUS4_HUMAN 242 9 YKCIPVEDNH DUS4_RAT 243 9 YKSIPVEDNQ DUS2_MOUSE 223 9 YKSIPVEDNQ DUS2_HUMAN 219 9 FLRVPVNDSF Q920R2 206 10 FLRVPVNDSF Q96N49 206 10 FLRVPVNDSF Q99MG5 151 10 FLRVPVNDSF Q99MG6 206 10 FLRVPVNDSF Q9AG15 151 10 FLRVPVNDSF Q9AG16 206 10 YKQIPISDHW DUS9_HUMAN 252 11 YKQIPISDHW DUS6_HUMAN 255 11 YKQIPISDHW DUS6_RAT 255 11 YKQIPISDHW Q99KC2 304 11 YKWIPVEDSH DUS5_HUMAN 225 9 YKWIPVEDSH DUS5_RAT 225 9 FMRIPINDNY DUS8_MOUSE 208 10 YKQIPISDHW Q91663 252 11 FMRVPINDNY DUS8_HUMAN 208 10 YKQIPISDHW Q8UW48 255 11 YKRLPATDSN Q9CZY9 371 11 YKRLPATDSN Q9ESS0 371 11 MAPKPHPHTASE5 Length of motif = 13 Motif number = 5 MAP kinase phosphatase motif V - 2 PCODE ST INT WFNEAIDFIDSIK DUS1_HUMAN 236 6 WFNEAIDFIDSIK DUS1_MOUSE 236 6 WFNEAIDFIDSIK DUS1_RAT 236 6 WFNEAIDFIDSIK Q63683 236 6 WFNEAIDFIDSIK Q64193 236 6 WFNEAIDFIDSIK Q90W58 238 6 WFNEAIDFIDSVK Q91790 238 6 WFMEAIEYIDSVK DUS4_CHICK 239 6 WFMEAIEYIDAVK DUS4_HUMAN 258 6 WFMEAIEYIDAVK DUS4_RAT 259 6 WFQEAISFIDSVK DUS2_MOUSE 239 6 WFQEAIGFIDWVK DUS2_HUMAN 235 6 WLDKSVDFIEKAK Q920R2 222 6 WLDKSVDFIEKAK Q96N49 222 6 WLDKSVDFIEKAK Q99MG5 167 6 WLDKSVDFIEKAK Q99MG6 222 6 WLDKSVDFIEKAK Q9AG15 167 6 WLDKSVDFIEKAK Q9AG16 222 6 FFPEAIEFIDEAL DUS9_HUMAN 268 6 FFPEAISFIDEAR DUS6_HUMAN 271 6 FFPEAISFIDEAR DUS6_RAT 271 6 FFPEAIAFIDEAL Q99KC2 320 6 HFQEAIDFIDCVR DUS5_HUMAN 241 6 HFQEAIDFIDCVR DUS5_RAT 241 6 WLDKSIEFIDKAK DUS8_MOUSE 224 6 FFPEAISFIDEAR Q91663 268 6 WLDKSIEFIDKAK DUS8_HUMAN 224 6 FFPEAIGFIDEAR Q8UW48 271 6 YFEEAFEFIEEAH Q9CZY9 387 6 YFEEAFEFIEEAH Q9ESS0 387 6

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