WORKLIST ENTRIES (1):

EAGCHANNEL View alignment       EAG potassium channel signature
 Type of fingerprint: COMPOUND with 12 elements
Links:
   PRINTS; PR00169 KCHANNEL; PR01463 EAGCHANLFMLY; PR01465 ELKCHANNEL
   PRINTS; PR01470 ERGCHANNEL

 Creation date 01-DEC-2000

   1. MILLER, C.
   An overview of the potassium channel family.
   GENOME BIOL. 1(4) 1-5 (2000).

   2. ASHCROFT, F.M.
   Voltage-gated K+ channels.
   IN ION CHANNELS AND DISEASE, ACADEMIC PRESS, 2000, PP.97-123.

   3. LITTLETON, J.T. AND GANETZKY, B.
   Ion Channels and synaptic organisation: analysis of the Drosophila genome	
   NEURON 26 35-43 (2000).

   4. FRINGS, S., BRULL, N., DZEJA, C. ANGELE, A., HAGEN, V. AND KAUPP U.B.
   Characterisation of a ether-a-go-go channels present in photoreceptors
   reveals similarity to IK a K+ current in rod inner segments.
   J.GEN.PHYSIOL. 111 583-599 (1998).

   Potassium ion (K+) channels are a structurally diverse group of proteins
   that facilitate the flow of K+ ions across cell membranes. They are
   ubiquitous, being present in virtually all cell types. Activation of K+
   channels tends to hyperpolarise cells, reducing the membrane's electrical
   resistance, dampening nervous activity. In eukaryotic cells, K+ channels
   are involved in neural signalling and generation of the cardiac rhythm, and
   act as effectors in signal transduction pathways involving G protein-
   coupled receptors (GPCRs). In prokaryotic cells, they play a role in the
   maintenance of ionic homeostasis [1].
  
   Structurally, EAG channels belong to the subfamily of K+ channels whose
   subunits contain 6 transmembrane (TM) domains: these are the voltage-gated
   K+ channels, the KCNQ channels, the EAG-like K+ channels and 3 kinds of
   Ca2+-activated K+ channel (BK, IK and SK) [2]. All K+ channels share a
   characteristic sequence feature: a TMxTVGYG motif that resides between the
   2 C-terminal membrane-spanning helices, and forms the K+-selective pore 
   domain [1]. However, unlike other families within this structural class,
   EAG channels possess a cyclic nucleotide binding domain within their 
   putative intracellular C-termini.
  
   The first EAG K+ channel was identified in Drosophila, following a screen 
   for mutations giving rise to behavioural abnormalities. Disruption of the
   Eag gene caused an ether-induced, leg-shaking behaviour. Subsequent studies
   have revealed a conserved multi-gene family of EAG-like K+ channels, which
   are present in Homo sapiens and many other species. Based on the varying
   functional properties of the channels, the family has been divided into
   3 subfamilies: EAG, ELK and ERG. Interestingly, C.elegans appears to lack
   the ELK type [3].
   
   The EAG subfamily has been expressed in heterologous systems to reveal their
   biophysical and pharmacological functions and to determine their currents in
   native tissues. All mammalian EAG subfamily K+ channels that have been 
   identified have properties typical of delayed rectifiers, with no measurable
   inactivation [5]. Only the Drosophila Eag channel exhibits partial
   inactivation.
  
   EAGCHANNEL is a 12-element fingerprint that provides a signature for the
   EAG potassium channels. The fingerprint was derived from an initial
   alignment of 7 sequences: the motifs were drawn from conserved regions
   spanning virtually the full alignment length, focusing on those sections
   that characterise the EAG channels but distinguish them from other members
   of the K+ channel superfamily - motifs 1-4 reside within the N-terminal 
   domain; motif 5 encodes putative TM domain 1; motif 6 encompasses TM domain 
   3; motif 7 spans part of the loop between TM domains 3 and 4; motif 8 spans
   the loop between TM domains 5 and 6; and motifs 9-12 reside within the 
   C-terminal region. Four iterations on SPTR39_14f were required to reach 
   convergence, at which point a true set comprising 9 sequences was
   identified. A single partial match was found, Q9XYX7, the C.elegans EAG K+ 
   channel EGL-2, which fails to make significant matches with motifs 1, 2, 4,
   8 and 12.

  SUMMARY INFORMATION
      9 codes involving 12 elements
      0 codes involving 11 elements
      0 codes involving 10 elements
      0 codes involving  9 elements
      0 codes involving  8 elements
      1 codes involving  7 elements
      0 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
   12|   9    9    9    9    9    9    9    9    9    9    9    9  
   11|   0    0    0    0    0    0    0    0    0    0    0    0  
   10|   0    0    0    0    0    0    0    0    0    0    0    0  
    9|   0    0    0    0    0    0    0    0    0    0    0    0  
    8|   0    0    0    0    0    0    0    0    0    0    0    0  
    7|   0    0    1    0    1    1    1    0    1    1    1    0  
    6|   0    0    0    0    0    0    0    0    0    0    0    0  
    5|   0    0    0    0    0    0    0    0    0    0    0    0  
    4|   0    0    0    0    0    0    0    0    0    0    0    0  
    3|   0    0    0    0    0    0    0    0    0    0    0    0  
    2|   0    0    0    0    0    0    0    0    0    0    0    0  
   --+-------------------------------------------------------------
     |   1    2    3    4    5    6    7    8    9   10   11   12  

True positives..
 O18965         Q63472         Q60603         O95259         
 O76035         O18966         Q9QXT2         Q9VXZ6         
 CIKE_DROME     
Subfamily:  Codes involving 7 elements
 Subfamily True positives..
 Q9XYX7         


  PROTEIN TITLES
   O18965           EAG CHANNEL - Bos taurus (Bovine).
   Q63472           POTASSIUM CHANNEL SUBUNIT - Rattus norvegicus (Rat).
   Q60603           LONG (ELECTOCARDIOGRAPHIC) QT SYNDROME 2 (POTASSIUM CHANNEL 
   O95259           VOLTAGE-GATED POTASSIUM CHANNEL EAGB - Homo sapiens (Human).
   O76035           POTASSIUM CHANNEL H-EAG - Homo sapiens (Human).
   O18966           EAG CHANNEL - Bos taurus (Bovine).
   Q9QXT2           POTASIUM CHANNEL EAG2 - Rattus norvegicus (Rat).
   Q9VXZ6           EAG PROTEIN - Drosophila melanogaster (Fruit fly).
   CIKE_DROME       POTASSIUM CHANNEL PROTEIN EAG - Drosophila melanogaster (Fru
 
   Q9XYX7           EAG K+ CHANNEL EGL-2 - Caenorhabditis elegans.

SCAN HISTORY SPTR39_14f 4 10 NSINGLE INITIAL MOTIF SETS EAGCHANNEL1 Length of motif = 8 Motif number = 1 EAG potassium channel motif I - 1 PCODE ST INT ENIIRRSN CIKE_DROME 17 17 ENIVRRSN O18966 19 19 ENIVRRSN O18965 19 19 ENIVRRSN O95259 19 19 ENIVRRSN Q60603 19 19 ENIVRRSN O76035 19 19 ENIVRRSN Q63472 19 19 EAGCHANNEL2 Length of motif = 14 Motif number = 2 EAG potassium channel motif II - 1 PCODE ST INT APIRNERDLVVLFL CIKE_DROME 134 109 APIRNEQDKVVLFL O18966 114 87 APIRNEQDKVVLFL O18965 114 87 APIRNEQDKVVLFL O95259 114 87 APIRNEQDKVVLFL Q60603 114 87 APIRNEQDKVVLFL O76035 114 87 APIRNEQDKVVLFL Q63472 114 87 EAGCHANNEL3 Length of motif = 12 Motif number = 3 EAG potassium channel motif III - 1 PCODE ST INT TFRDITALKQPI CIKE_DROME 149 1 TFSDITAFKQPI O18966 129 1 TFSDITAFKQPI O18965 129 1 TFSDITAFKQPI O95259 129 1 TFSDITAFKQPI Q60603 129 1 TFSDITAFKQPI O76035 129 1 TFSDITAFKQPI Q63472 129 1 EAGCHANNEL4 Length of motif = 13 Motif number = 4 EAG potassium channel motif IV - 1 PCODE ST INT KFAKLARSVTRSR CIKE_DROME 173 12 KFARLTRALTSSR O18966 150 9 KFARLTRALTSSR O18965 150 9 KFARLTRALTSSR O95259 150 9 KFARLTRALTSSR Q60603 150 9 KFARLTRALTSSR O76035 150 9 KFARLTRALTSSR Q63472 150 9 EAGCHANNEL5 Length of motif = 18 Motif number = 5 EAG potassium channel motif V - 1 PCODE ST INT SLSADIMPQYRQEAPKTP CIKE_DROME 208 22 QLGSDILPQYKQEAPKTP O18966 189 26 QLGSDILPQYKQEAPKTP O18965 189 26 QLGSDILPQYKQEAPKTP O95259 189 26 QLGSDILPQYKQEAPKTP Q60603 189 26 QLGSDILPQYKQEAPKTP O76035 189 26 QLGSDILPQYKQEAPKTP Q63472 189 26 EAGCHANNEL6 Length of motif = 9 Motif number = 6 EAG potassium channel motif VI - 1 PCODE ST INT IIDLLSCLP CIKE_DROME 317 91 VIDLLSCLP O18966 297 90 VIDLLSCLP O18965 297 90 VIDLLSCLP O95259 297 90 VIDLLSCLP Q60603 297 90 VIDLLSCLP O76035 297 90 VIDLLSCLP Q63472 297 90 EAGCHANNEL7 Length of motif = 10 Motif number = 7 EAG potassium channel motif VII - 1 PCODE ST INT GIGSLFSALK CIKE_DROME 338 12 GISSLFSSLK O18966 345 39 GISSLFSSLK O18965 318 12 GISSLFSSLK O95259 345 39 GISSLFSSLK Q60603 345 39 GISSLFSSLK O76035 318 12 GISSLFSSLK Q63472 318 12 EAGCHANNEL8 Length of motif = 7 Motif number = 8 EAG potassium channel motif VIII - 1 PCODE ST INT SWLWKLA CIKE_DROME 405 57 SWLYQLA O18966 417 62 SWLYQLA O18965 390 62 SWLYQLA O95259 417 62 SWLYQLA Q60603 417 62 SWLYQLA O76035 390 62 SWLYQLA Q63472 390 62 EAGCHANNEL9 Length of motif = 13 Motif number = 9 EAG potassium channel motif IX - 1 PCODE ST INT TEKVLNCCPKDMK CIKE_DROME 542 130 TEKVLQICPKDMR O18966 552 128 TEKVLQICPKDMR O18965 525 128 TEKVLQICPKDMR O95259 552 128 TEKVLQICPKDMR Q60603 552 128 TEKVLQICPKDMR O76035 525 128 TEKVLQICPKDMR Q63472 525 128 EAGCHANNEL10 Length of motif = 16 Motif number = 10 EAG potassium channel motif X - 1 PCODE ST INT DFYSAFANSFARNLVL CIKE_DROME 672 117 EFYTAFSHSFSRNLIL O18966 682 117 EFYTAFSHSFSRNLIL O18965 655 117 EFYTAFSHSFSRNLIL O95259 682 117 EFYTAFSHSFSRNLIL Q60603 682 117 EFYTAFSHSFSRNLIL O76035 655 117 EFYTAFSHSFSRNLIL Q63472 655 117 EAGCHANNEL11 Length of motif = 15 Motif number = 11 EAG potassium channel motif XI - 1 PCODE ST INT RHRLIFRRVADVKRE CIKE_DROME 692 4 RKRIVFRKISDVKRE O18966 702 4 RKRIVFRKISDVKRE O18965 675 4 RKRIVFRKISDVKRE O95259 702 4 RKRIVFRKISDVKRE Q60603 702 4 RKRIVFRKISDVKRE O76035 675 4 RKRIVFRKISDVKRE Q63472 675 4 EAGCHANNEL12 Length of motif = 12 Motif number = 12 EAG potassium channel motif XII - 1 PCODE ST INT DHLVRKIFSKFR CIKE_DROME 724 17 DHPVRRLFQRFR O18966 734 17 DHPVRRLFQRFR O18965 707 17 DHPVRRLFQRFR O95259 734 17 DHPVRRLFQRFR Q60603 734 17 DHPVRRLFQRFR O76035 707 17 DHPVRRLFQRFR Q63472 707 17 FINAL MOTIF SETS EAGCHANNEL1 Length of motif = 8 Motif number = 1 EAG potassium channel motif I - 4 PCODE ST INT ENIVRRSN O18965 19 19 ENIVRRSN Q63472 19 19 ENIVRRSN Q60603 19 19 ENIVRRSN O95259 19 19 ENIVRRSN O76035 19 19 ENIVRRSN O18966 19 19 ENIVRRSS Q9QXT2 17 17 ENIIRRSN Q9VXZ6 17 17 ENIIRRSN CIKE_DROME 17 17 EAGCHANNEL2 Length of motif = 14 Motif number = 2 EAG potassium channel motif II - 4 PCODE ST INT APIRNEQDKVVLFL O18965 114 87 APIRNEQDKVVLFL Q63472 114 87 APIRNEQDKVVLFL Q60603 114 87 APIRNEQDKVVLFL O95259 114 87 APIRNEQDKVVLFL O76035 114 87 APIRNEQDKVVLFL O18966 114 87 APIRNEHEKVVLFL Q9QXT2 112 87 APIRNERDLVVLFL Q9VXZ6 134 109 APIRNERDLVVLFL CIKE_DROME 134 109 EAGCHANNEL3 Length of motif = 12 Motif number = 3 EAG potassium channel motif III - 4 PCODE ST INT TFSDITAFKQPI O18965 129 1 TFSDITAFKQPI Q63472 129 1 TFSDITAFKQPI Q60603 129 1 TFSDITAFKQPI O95259 129 1 TFSDITAFKQPI O76035 129 1 TFSDITAFKQPI O18966 129 1 TFKDITLFKQPI Q9QXT2 127 1 TFRDITALKQPI Q9VXZ6 149 1 TFRDITALKQPI CIKE_DROME 149 1 EAGCHANNEL4 Length of motif = 13 Motif number = 4 EAG potassium channel motif IV - 4 PCODE ST INT KFARLTRALTSSR O18965 150 9 KFARLTRALTSSR Q63472 150 9 KFARLTRALTSSR Q60603 150 9 KFARLTRALTSSR O95259 150 9 KFARLTRALTSSR O76035 150 9 KFARLTRALTSSR O18966 150 9 KFARLTRALTNSR Q9QXT2 148 9 KFAKLARSVTRSR Q9VXZ6 173 12 KFAKLARSVTRSR CIKE_DROME 173 12 EAGCHANNEL5 Length of motif = 18 Motif number = 5 EAG potassium channel motif V - 4 PCODE ST INT QLGSDILPQYKQEAPKTP O18965 189 26 QLGSDILPQYKQEAPKTP Q63472 189 26 QLGSDILPQYKQEAPKTP Q60603 189 26 QLGSDILPQYKQEAPKTP O95259 189 26 QLGSDILPQYKQEAPKTP O76035 189 26 QLGSDILPQYKQEAPKTP O18966 189 26 QLGSDILPQYKQEAPKTP Q9QXT2 186 25 SLSADIMPQYRQEAPKTP Q9VXZ6 208 22 SLSADIMPQYRQEAPKTP CIKE_DROME 208 22 EAGCHANNEL6 Length of motif = 9 Motif number = 6 EAG potassium channel motif VI - 4 PCODE ST INT VIDLLSCLP O18965 297 90 VIDLLSCLP Q63472 297 90 VIDLLSCLP Q60603 297 90 VIDLLSCLP O95259 297 90 VIDLLSCLP O76035 297 90 VIDLLSCLP O18966 297 90 VIDLLSCLP Q9QXT2 294 90 IIDLLSCLP Q9VXZ6 317 91 IIDLLSCLP CIKE_DROME 317 91 EAGCHANNEL7 Length of motif = 10 Motif number = 7 EAG potassium channel motif VII - 4 PCODE ST INT GISSLFSSLK O18965 318 12 GISSLFSSLK Q63472 318 12 GISSLFSSLK Q60603 345 39 GISSLFSSLK O95259 345 39 GISSLFSSLK O76035 318 12 GISSLFSSLK O18966 345 39 GISSLFSSLK Q9QXT2 315 12 GIGSLFSALK Q9VXZ6 338 12 GIGSLFSALK CIKE_DROME 338 12 EAGCHANNEL8 Length of motif = 7 Motif number = 8 EAG potassium channel motif VIII - 4 PCODE ST INT SWLYQLA O18965 390 62 SWLYQLA Q63472 390 62 SWLYQLA Q60603 417 62 SWLYQLA O95259 417 62 SWLYQLA O76035 390 62 SWLYQLA O18966 417 62 SWLYQLA Q9QXT2 387 62 SWLWKLA Q9VXZ6 405 57 SWLWKLA CIKE_DROME 405 57 EAGCHANNEL9 Length of motif = 13 Motif number = 9 EAG potassium channel motif IX - 4 PCODE ST INT TEKVLQICPKDMR O18965 525 128 TEKVLQICPKDMR Q63472 525 128 TEKVLQICPKDMR Q60603 552 128 TEKVLQICPKDMR O95259 552 128 TEKVLQICPKDMR O76035 525 128 TEKVLQICPKDMR O18966 552 128 TEKVLSICPKDMR Q9QXT2 521 127 TEKVLNYCPKDMK Q9VXZ6 542 130 TEKVLNCCPKDMK CIKE_DROME 542 130 EAGCHANNEL10 Length of motif = 16 Motif number = 10 EAG potassium channel motif X - 4 PCODE ST INT EFYTAFSHSFSRNLIL O18965 655 117 EFYTAFSHSFSRNLIL Q63472 655 117 EFYTAFSHSFSRNLIL Q60603 682 117 EFYTAFSHSFSRNLIL O95259 682 117 EFYTAFSHSFSRNLIL O76035 655 117 EFYTAFSHSFSRNLIL O18966 682 117 DFYTAFANSFSRNLTL Q9QXT2 651 117 DFYSAFANSFARNLVL Q9VXZ6 672 117 DFYSAFANSFARNLVL CIKE_DROME 672 117 EAGCHANNEL11 Length of motif = 15 Motif number = 11 EAG potassium channel motif XI - 4 PCODE ST INT RKRIVFRKISDVKRE O18965 675 4 RKRIVFRKISDVKRE Q63472 675 4 RKRIVFRKISDVKRE Q60603 702 4 RKRIVFRKISDVKRE O95259 702 4 RKRIVFRKISDVKRE O76035 675 4 RKRIVFRKISDVKRE O18966 702 4 RKRIIFRKISDVKKE Q9QXT2 671 4 RHRLIFRKVADVKRE Q9VXZ6 692 4 RHRLIFRRVADVKRE CIKE_DROME 692 4 EAGCHANNEL12 Length of motif = 12 Motif number = 12 EAG potassium channel motif XII - 4 PCODE ST INT DHPVRRLFQRFR O18965 707 17 DHPVRRLFQRFR Q63472 707 17 DHPVRRLFQRFR Q60603 734 17 DHPVRRLFQRFR O95259 734 17 DHPVRRLFQRFR O76035 707 17 DHPVRRLFQRFR O18966 734 17 DHPVRKLFQKFK Q9QXT2 703 17 DHLVRKIFSKFR Q9VXZ6 724 17 DHLVRKIFSKFR CIKE_DROME 724 17

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